The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells and has been described in detail in OECD TG 487. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable

Last updated on: 05-11-2021 - 15:53

Contact: Birgit Mertens
Organisation:
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The method is described in detail in OECD TG 439 as SkinEthicTM RHE and provides an in vitro procedure that may be used for the hazard identification of irritant chemicals (substances and mixtures) in accordance with the United Nations Globally Harmonized System of Classification and Labelling (UN

Last updated on: 11-03-2021 - 19:17

Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
The ERα CALUX transactivation assay is described in Annex 4 of the OECD TG 455. It uses the human U2OS cell line to detect estrogenic agonist and antagonist activity mediated through human estrogen receptor alpha (hERα).This test method is specifically designed to detect hERα-mediated

Last updated on: 04-03-2021 - 16:33

Contact: Cannot be disclosed
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
The method has been fully described in OECD TG 236 and is intended to determine the acute or lethal toxicity of chemicals on embryonic stages of fish (Danio rerio). Newly fertilized zebrafish eggs are exposed to the test chemical for a period of 96 hrs. Every 24 hrs. Twenty embryos (one embryo per

Last updated on: 04-03-2021 - 16:29

Contact: Hilda Witters
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The Bovine Corneal Opacity and Permeability test method (BCOP) has been fully described in OECD TG 437. It is an in vitro test method that can be used to identify chemicals (substances or mixtures) as either 1) causing “serious eye damage” (category 1 of the Globally Harmonised System for the

Last updated on: 04-03-2021 - 16:26

Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
Four validated test methods using commercially available RhCE models are included in this Test Guideline 492. Here we focus on the SkinEthic Human Corneal Epithelium (HCE) Eye Irritation Test (EIT). It is an in vitro procedure allowing the identification of chemicals (substances and mixtures) not

Last updated on: 04-03-2021 - 16:19

Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
This method has been described in detail in OECD TG 431 and allows the identification of non-corrosive and corrosive substances and mixtures in accordance with the UN GHS. This method further supports the sub-categorisation of corrosive substances and mixtures into optional Subcategory 1A, in

Last updated on: 04-03-2021 - 16:15

Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
In order to find a solution for the center-weighted opacity reading associated with the OP-KIT opacitometer, a prototype of a laser light-based opacitometer (PLLBO) allowing better measurement of opacities was developed (Van Goethem et al., 2010; Annex 1). The technical optimization and optical

Last updated on: 04-03-2021 - 16:05

Contact: An Van Rompay
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
This is a mathematical compartmental formulation of dose-effect synergy modelling for multiple therapies in Non Small Cell Lung Cancer (NSCLC): antiangiogenic, immuno- and radiotherapy. The model formulates the dose-effect relationship in a unified context, with tumor proliferating rates and

Last updated on: 01-02-2021 - 14:32

Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Experiments were conducted using cortical cells that contains glutamatergic and gabaergic neurons and glia. Primary neurons were freshly dissociated from embryonic E18-19 rat cortices as described previously and plated onto 48-well MEA plates (Maestro system, Axion Biosystems). HOne day before

Last updated on: 26-01-2021 - 17:27

Contact: Cannot be disclosed
Organisation: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal