The maturation of the human brain shows species-specific differences of neoteny when compared to lower mammals. This process encompass a time window that expands from late embryonic stages to early adolescence. Major features of brain maturation are the acquisition of phenotypic complex traits such

Last updated on: 25-09-2023 - 14:38

Organisation: University of Liège (ULiège)
Status: Still in development, History of use
The method is an approach to systematically study the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells, based on performing stable 13C isotope labeling measurements on in vitro-differentiated murine effector CD8+ T cells. Naive CD8+ T cells are isolated from mouse

Last updated on: 31-08-2023 - 17:12

Contact: Dorien Broekaert
Organisation: Katholieke Universiteit Leuven (KUL), VIB - KU Leuven
Status: Published in peer reviewed journal
Metabolomics and 13C tracer analysis are state-of-the-art techniques that allow determining the concentration of metabolites and the activity of metabolic pathways, respectively. Three dimensional (3D) cultures of cancer cells constitute an enriched in vitro environment that can be used to assay

Last updated on: 31-08-2023 - 17:12

Contact: Dorien Broekaert
Organisation: VIB - KU Leuven, Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
This assay evaluates the sprouting ability of endothelial cells in a collagen matrix and it used as an in vitro model to study the formation of new blood/lymphatic vessels. The effect of pro-angiogenic growth factors or co-cultured cells can be measured by quantifying the amount of vascular sprouts

Last updated on: 25-08-2023 - 09:11

Contact: Diede Houbaert
Organisation: Cellular and Molecular Medicine - KU Leuven
Status: History of use, Internally validated, Published in peer reviewed journal
The use of patient-derived primary cell cultures in cancer preclinical assays, including drug screens and genotoxic studies, has increased in recent years. However, their translational value is constrained by several limitations, including variability that can be caused by the culture conditions.

Last updated on: 20-07-2023 - 15:05

Contact: Ahmad Najem
Organisation: Institut Jules Bordet
Status: Published in peer reviewed journal
The Systemic Intestinal Fermentation Research technology, SIFR in short, is a uniquely validated simulation of gut microbial ecosystems, shown to be predictive for clinical outcomes. The SIFR can simulate a wide variety of gut microbiota from humans (infants, adults, elderly; healthy, diseased) to

Last updated on: 08-06-2023 - 14:24

Contact: Aurélien Baudot
Organisation: Cryptobiotix
Status: History of use, Internally validated, Published in peer reviewed journal
Using an in vitro flow system adhesion assay, immune cell adhesion to (blood-brain barrier) endothelial cells can be quantified under physiological blood flow conditions. Endothelial cells can be treated with inflammatory cytokines or therapeutic antibodies to mimic inflammatory diseases. Immune

Last updated on: 06-06-2023 - 10:35

Contact: Doryssa Hermans
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal
ProDigest's short-term colonic simulation is an in vitro model for rapid screening of the interaction between test products and the gut microbiome. The key microbial saccharolytic and proteolytic markers are analysed as well as evolution in the composition of the microbiome and other endpoints as

Last updated on: 17-04-2023 - 13:22

Contact: Cannot be disclosed
Organisation: ProDigest
Partners: ProDigest
Status: History of use, Internally validated, Published in peer reviewed journal
Macrophages orchestrate reactive particle segregation, compact aggregates of immune cells and non-immune cells and promote fibrosis-surrounding granulomas. We developed a simple 3D in-vitro model that mimics granuloma formation and categorizes granuloma-inducing inorganic particles. Macrophage

Last updated on: 03-04-2023 - 11:21

Contact: Léa Hiéronimus
Organisation: Université Catholique de Louvain (UCL)
Status: Still in development, Internally validated
We aim to differentiate ovarian cells isolated from postmenopausal ovaries into steroidogenic theca interna cells. For this purpose, cryopreserved ovarian cortex fragments are used to isolate ovarian cells. These cells are cultured on collagen and supplemented with growth factors defined to promote

Last updated on: 20-03-2023 - 13:52

Contact: Hanne Vlieghe
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal