Using the ex vivo culture system, we investigated the impact of an endoscopic helium plasma jet application on mouse ISCs at the morphological, cellular and transcriptomic levels. Moreover, we explored the potential selectivity of CAP application on tumor versus normal organoids originating from the

Last updated on: 09-01-2023 - 09:42

Organisation: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
The tri-dimensional (3D) culture protocols allow isolation and culture of gastric epithelial antral and intestinal stem cells to efficiently generate organoids that recapitulate the mature pyloric and intestinal epithelium in vitro and foetal epithelial progenitors of these tissues growing as

Last updated on: 16-08-2022 - 10:57

Organisation: Université Libre de Bruxelles (ULB)
Status: History of use, Internally validated, Published in peer reviewed journal
Alkaline comet assay (COM) and Micronucleus test (MN) are well-established cytogenetic tests that are used to detect both immediate damages i.e. DNA fragmentation, and permanent damage which results in micronuclei formation. These techniques are often applied separately or, sometimes, in combination

Last updated on: 18-02-2022 - 16:42

Contact: Ha Nguyen
Organisation: Université Libre de Bruxelles (ULB), Sciensano
Partners: University of Liège (ULiège)
Status: History of use, Internally validated
We used a 7-stage protocol to generate beta cells from human Induced Pluripotent Stem Cells (iPSC) and evaluated whether these cells are responsive to the pro-inflammatory cytokines (IFNγ, IL-1β, or IFNα) that play a role in type 1 diabetes (T1D). Our data show that human iPSC-derived beta cells

Last updated on: 09-03-2021 - 21:30

Contact: Decio L. Eizirik
Organisation: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
Integrated approach for the characterization,validation and monitoring of protein biomarkers in animal tissue samples as well as on human tissue samples. The ''cell-block'' technique allows the study of cell lines with the same approach. The methodology involves histological and standardized

Last updated on: 12-11-2019 - 11:37

Contact: Cédric Balsat
Organisation: Université Libre de Bruxelles (ULB)
Partners: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal