Macrophages orchestrate reactive particle segregation, compact aggregates of immune cells and non-immune cells and promote fibrosis-surrounding granulomas.
We developed a simple 3D in-vitro model that mimics granuloma formation and categorizes granuloma-inducing inorganic particles. Macrophage
Last updated on: 03-04-2023 - 11:21
Contact: Léa Hiéronimus
Organisation: Université Catholique de Louvain (UCL)
Status: Still in development, Internally validated
We aim to differentiate ovarian cells isolated from postmenopausal ovaries into steroidogenic theca interna cells. For this purpose, cryopreserved ovarian cortex fragments are used to isolate ovarian cells. These cells are cultured on collagen and supplemented with growth factors defined to promote
Last updated on: 20-03-2023 - 13:52
Contact: Hanne Vlieghe
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
The goal of chicken embryonic spinal cord electroporation is to increase or to reduce expression levels of genes of interest in the developing spinal cord, and to assess the phenotypic consequences of these alterations on neuronal differentiation or migration. Fertilized eggs stored at 14°C are
Last updated on: 13-12-2022 - 11:23
Contact: Frédéric Clotman
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
This method describes the generation of porcine testicular organoids using piglet testicular cells seeded in a testicular extracellular matrix (tECM) hydrogel. To generate the solublized tECM hydrogel, porcine immature testicular tissues (ITTs) were dissected in small fragments and decellularized in
Last updated on: 04-11-2022 - 08:20
Contact: Marc Kanbar
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
We aimed to optimize fibrin matrix composition in order to mimic human ovarian tissue architecture for human ovarian follicle encapsulation and grafting. Ultrastructure of fresh human ovarian cortex in age-related women (n = 3) and different fibrin formulations (F12.5/T1, F30/T50, F50/T50, F75/T75),
Last updated on: 10-09-2021 - 14:18
Contact: Christiani Amorim
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
We developed an innovative model to study the effect of the supernumerary X chromosome on KS features. The model was generated using induced pluripotent stem cells (iPSCs) from patients with Klinefelter syndrome (KS) i.e. with a 47, XXY karyotype. In order to compare the potentials of both 47XXY
Last updated on: 07-01-2021 - 21:23
Contact: Christine Wyns
Organisation: Université Catholique de Louvain (UCL)
Partners: Geneva University Hospitals
Status: Published in peer reviewed journal
The ex-vivo endothelium-stent contact model allows measuring the impact of implants on the arterial tissue. The endothelium is directly exposed by inserting a wire made of the investigated material into the lumen of the aortic ring to mimic the contact between the coronary stent and the endothelium.
Last updated on: 03-12-2019 - 17:23
Contact: Eleonora Scarcello
Organisation: Université Catholique de Louvain (UCL)
Partners: Université Catholique de Louvain (UCL)
Status: Internally validated
The cytokinesis-block micronucleus method allows assessing the presence of DNA damage at the chromosome level. It is an essential part of toxicology, because mutation is a crucial event in carcinogenesis. The capacity of inhaled particles to induce irreversible mutations in type II lung epithelial
Last updated on: 26-11-2019 - 17:35
Contact: Violaine Sironval
Organisation: Université Catholique de Louvain (UCL)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
The hemolysis assay remarkably predicts the inflammatory potential of inhaled particles. The capacity of particles to damage cellular membranes is a key property to predict their inflammatory potential upon inhalation. In macrophages and epithelial cells exposed to particles, alteration of the
Last updated on: 15-11-2019 - 16:15
Contact: Dominique Lison
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
Hyper Polarized (HP) MR allows to considerably increase the sensitivity (>10.000) of MR spectroscopy and spectroscopic imaging. The hyperpolarized molecule can be injected at room temperature to a cell system or an in vivo model and its metabolism can be followed over a few minutes.The technique
Last updated on: 08-11-2019 - 13:56
Contact: Bénédicte Jordan
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
