This method provides a rapid untargeted screening metabolomics method, which can be used to evaluate whether or not metabolic changes can be appointed according to the metabolic state in your matrix of choice. It applies an ambient ionization technique in which a laser beam or diathermy probe is

Last updated on: 31-03-2022 - 15:31

Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
The in vitro rumen fermentation technique is used to simulate the rumen function/fermentation. The rumen is the first compartment of the digestive tract of ruminants, in which feed - consumed by ruminants - is fermented by rumen microbes. For optimal fermentation, the rumen provides an anaerobic

Last updated on: 07-03-2022 - 13:26

Contact: Veerle Fievez
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase

Last updated on: 11-02-2022 - 10:29

Contact: Olivier De Wever
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Advances in cell culture methods allowed 3D culture of gut epithelial cells derived from adult intestinal epithelial stem cells in mice and humans. We show that starting from crypts cultured in an extracellular matrix hydrogel together with essential growth factors, gut organoids can be grown from

Last updated on: 20-01-2022 - 16:14

Contact: Bert Devriendt
Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
The response of sublingual epithelial cells to house dust mite allergen and potential tolerance-promoting adjuvants such as Toll-like receptor (TLR) ligands and calcitriol was investigated using primary sublingual epithelial cells isolated from dogs and cultured in vitro. After 24-h incubation with

Last updated on: 07-04-2021 - 14:52

Contact: Eric Cox
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
This is a mathematical compartmental formulation of dose-effect synergy modelling for multiple therapies in Non Small Cell Lung Cancer (NSCLC): antiangiogenic, immuno- and radiotherapy. The model formulates the dose-effect relationship in a unified context, with tumor proliferating rates and

Last updated on: 01-02-2021 - 14:32

Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Computational Fluid Dynamics (CFD) is being applied to characterize the fluid flow in different applications. CFD has obtained significant interest in both the medical and engineering community because of its non-invasive character. It can predict the fluid flow characteristics when one or multiple

Last updated on: 10-04-2020 - 09:22

Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
This is a method to study host-microbe interaction in the upper respiratory tract. A physiologically representative epithelial structure, with mucin producing and ciliated cells, is obtained by culturing respiratory epithelial cells at air-liquid interface in Transwell inserts. Optionally,

Last updated on: 28-02-2020 - 10:51

Organisation: Ghent University (UGent)
Status: Still in development, History of use, Internally validated, Published in peer reviewed journal
It is the goal of the DNA-adductomics to search for DNA-adducts that might be formed during interaction with contaminants. The analysis of DNA adducts is performed using ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the

Last updated on: 12-11-2019 - 11:16

Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
Our polar metabolomics profiling and fingerprinting methodology applies ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the instrumental method, as well as generic extraction protocols for colon tissue, cell cultures, urine,

Last updated on: 12-11-2019 - 11:13

Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal