Cytotoxicity measurement in cultured primary rat hepatocytes

The MTT test is performed to determine the in vitro cytotoxicity of selected chemicals. The mitochondrial enzyme succinate deydrogenase is responsible for the biotransformation of toxic agents and MTT. The ability of cells to reduce MTT provides an indication of the mitochondrial integrity and

Last updated on: 08-11-2019 - 09:14

Contact: Axelle Cooreman
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
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Measurement of the extracellular release of adenosine triphosphate in cultured primary rat hepatocytes

ATP transports chemical energy within cells by serving as a substrate for kinases and as such fulfills a vital function in numerous cellular processes such as cell injury and subsequent cell death. ATP is therefore a crucial player in these events that are results of intracellular stress.

Last updated on: 08-11-2019 - 09:13

Contact: Axelle Cooreman
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
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Spheroid cell culture of human skin-derived precursors

For the generation of the human skin-derived precursors (hSKP) spheroids, ultra-low attachment 96-well plates, cell-repellent (Greiner) are used. hSKP cultured on tissue culture plates are detached with trypple (5 minutes) and collected in a tube. After centrifugation, hSKP are counted at the Burker

Last updated on: 08-11-2019 - 09:13

Contact: Alessandra Natale
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
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Manufacturing of fiber scaffolds and cell seeding

Polycaprolactone (PCL) fiber materials are fabricated using an electrospinning method. Molecular weight of PCL is 45 000 Da (Sigma-Aldrich). The electrospinning process is performed using 18wt% PCL solution dissolved in chloroform:ethanol at a ratio of 9:1. Two high-voltage sources are used to

Last updated on: 08-11-2019 - 09:13

Contact: Alessandra Natale
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
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Drug-induced phospholipidosis in hepatic cells derived from human skin-derived precursors

Drug-induced phospholipidosis (DIPL) is a metabolic disorder characterized by an excessive intracellular accumulation of phospholipids caused by cationic drugs. Hepatic cells derived from human skin are evaluated as an in vitro model to investigate DIPL and its mechanisms. Human skin stem cells

Last updated on: 08-11-2019 - 09:13

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
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Microfluidic perfusion culture for hepatic differentiation of human skin stem cells

The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP). In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In a

Last updated on: 08-11-2019 - 09:07

Contact: Alessandra Natale
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
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In vitro human stem cell-based model of non-alcoholic steatohepatitis

Non-alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to severe, life-threatening non-alcoholic steatohepatitis (NASH). Steatosis is mostly asymptomatic and does not cause health complications. However, in 5-10% of the cases it proceeds to NASH in which hepatic inflammation occurs

Last updated on: 08-11-2019 - 09:04

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
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Establishment of sandwich cultures of primary human hepatocytes

This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix interactions.

Last updated on: 08-11-2019 - 09:03

Contact: Eva Gijbels
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
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In vitro human experimental model for pancreatic acinar dedifferentiation

Loss of acinar differentiation drives pancreatic cancer. An established human in vitro experimental model is used in our lab to study this process. Pancreatic exocrine cells from human donors are placed in suspension culture in Advanced RPMI medium supplemented with 5% heat-inactivated fetal bovine

Last updated on: 21-08-2019 - 11:36

Contact: Elyne Backx
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
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