Mononuclear cells (MNC) are isolated from bone marrow aspirates by density gradient centrifugation and washed in Hank's buffered salt solution. MNC are seeded at a cell density of 2 × 10E4 cells/cm2 in low glucose DMEM supplemented with 15% (v/v) heat-inactivated FBS, 2 mM L-glutamine and 0.5% (v/v)
Last updated on: 08-11-2019 - 09:33
Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Approximately 125 g of processed adipose tissue is incubated for 90 minutes at 37°C in dissociation medium (1:1) consisting of 1% (v/v) bovine serum albumin and 1 mg/mL collagenase A in phosphate buffered saline (PBS). After two filtration steps, the filtrate is carefully brought on top of 15 mL of
Last updated on: 08-11-2019 - 09:32
Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Human skin-derived precursor cells (hSKP) between are cultivated at 90% confluency on 100 µg/mL collagen type I in base medium and sequentially exposed to hepatogenic factors. Base medium consists of Dulbecco’s Modified Eagle Medium + GLUTAMAX / F12 Nutrient supplement (3:1), supplemented with 7.33
Last updated on: 08-11-2019 - 09:31
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
E. coli is one of the organisms of choice for the production of recombinant proteins. DH5 alpha cells are commonly used for maintenance, propagation and mutation, whilst BL21(DE3) and C43(DE3) are mainly used for expression of the transgene. The advantage of C43(DE3) is that is used to produce
Last updated on: 08-11-2019 - 09:28
Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel
Status: Still in development, History of use, Internally validated
Freshly collected human foreskin samples are incubated with 0.2 mg/mL Liberase DH solution and incubated overnight at 4°C. The next day, the epidermis is removed and the tissue is incubated at 37°C for another 10-20 minutes, depending on the sample size. After processing the samples, typically 5 -
Last updated on: 08-11-2019 - 09:27
Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human cells
Last updated on: 08-11-2019 - 09:25
Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel
Status: Still in development, History of use
This method describes the steps to go from a liver to a decellularized matrix. It uses mild and strong detergents to destroy cells and keep the extracellular matrix intact. This matrix can then be used for a variety of purposes, including (but not limited to) repopulation, basis for coating and
Last updated on: 08-11-2019 - 09:24
Contact: Paul Claes
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal
This method describes the steps from a living rat to a single cell solution of primary hepatocytes. This requires surgery on the lab animal, a perfusion with buffer solution, a digestion with collagenase and a filtration step to obtain primary hepatocytes.
Last updated on: 08-11-2019 - 09:22
Human skin-derived adult stem cells differentiated towards hepatic cells (hSKP-HPC) are used in this method (R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014)). These cells are exposed to a cocktail of insulin and glucose at certain concentrations. After 24h of exposure, these cells exhibit a
Last updated on: 08-11-2019 - 09:19
Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line to
Last updated on: 08-11-2019 - 09:17
