The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP). In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In a

Last updated on: 08-11-2019 - 09:07

Organisation: Vrije Universiteit Brussel
Status: Still in development
Non-alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to severe, life-threatening non-alcoholic steatohepatitis (NASH). Steatosis is mostly asymptomatic and does not cause health complications. However, in 5-10% of the cases it proceeds to NASH in which hepatic inflammation occurs

Last updated on: 08-11-2019 - 09:04

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Still in development
This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix interactions.

Last updated on: 08-11-2019 - 09:03

Contact: Eva Gijbels
Organisation: Vrije Universiteit Brussel
Status: Still in development
Loss of acinar differentiation drives pancreatic cancer. An established human in vitro experimental model is used in our lab to study this process. Pancreatic exocrine cells from human donors are placed in suspension culture in Advanced RPMI medium supplemented with 5% heat-inactivated fetal bovine

Last updated on: 21-08-2019 - 11:36

Contact: Elyne Backx
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal