These are the general guidelines on sample input requirements to successfully start our RIBO-seq procedures: Cell lines in suspension: pellet of 10 – 50 M snap frozen cells. The pre-treatment and collection specifications are also mentioned in our pre-processing protocol. Adherent cell lines

Last updated on: 21-10-2022 - 10:33

Contact: Zarel Martinez
Organisation: OHMX.bio
Status: History of use, Internally validated, Published in peer reviewed journal
We have developed a protocol for culturing primary neuron progenitor cells (NPCs) derived from mouse embryos at embryonic day 15 (E15). These cells are highly proliferative, can be subcultured and cryopreserved and can be differentiated to neurons or astrocytes. Hence, this method can greatly reduce

Last updated on: 20-10-2022 - 08:55

Contact: Roel Quintens
Organisation: Belgian Nuclear Research Centre
Status: Still in development, History of use, Internally validated
The in vitro rumen fermentation technique is used to simulate the rumen function/fermentation. The rumen is the first compartment of the digestive tract of ruminants, in which feed - consumed by ruminants - is fermented by rumen microbes. For optimal fermentation, the rumen provides an anaerobic

Last updated on: 14-10-2022 - 11:37

Contact: Veerle Fievez
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The tri-dimensional (3D) culture protocols allow isolation and culture of gastric epithelial antral and intestinal stem cells to efficiently generate organoids that recapitulate the mature pyloric and intestinal epithelium in vitro and foetal epithelial progenitors of these tissues growing as

Last updated on: 16-08-2022 - 10:57

Organisation: Université Libre de Bruxelles (ULB)
Status: History of use, Internally validated, Published in peer reviewed journal
For therapeutic or reporter molecules to be effective for therapy or imaging applications, proper accumulation of the compounds in the tissue of interest is required, with minimal accumulation in undesired organs to avoid toxic side-effects and increase bioavailability. After initial in vitro

Last updated on: 09-08-2022 - 16:34

Contact: Sophie Hernot
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
The main objective of this research project is to create the first full-thickness cornea-on-chip, which comprises a 3D construct with every cellular layer of the cornea. This construct is embedded in a microfluidic chip with two channels that are continuously perfused. The epithelial side of the

Last updated on: 27-07-2022 - 09:35

Contact: Joris Van Meenen
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development
A battery of in vitro genotoxicity assays is currently used to detect agents with DNA damaging and carcinogenic potential. Although the sensitivity of this battery of genotoxicity assays is high, the specificity is low (“false positive hit”), especially with the mammalian cell-based assay, compared

Last updated on: 11-07-2022 - 15:51

Contact: Marc Audebert
Organisation: Institut national de la recherche agronomique (INRAE)
Status: History of use, Internally validated, Published in peer reviewed journal, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
In vitro differentiation of hematopoietic stem cells (HSC) or inducible pluripotent stem cells (IPS) to megakaryocytes and platelets using specific differentiation conditions (liquid and 3D media). CRISPR/cas mutagenesis of HSC or IPS to study the effect of gene depletion or specific mutants on

Last updated on: 26-05-2022 - 10:41

Contact: Kathleen Freson
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated, Published in peer reviewed journal
The VITOTOX test is a bacterial genotoxicity test. The test is based on bacteria that contain the lux operon of Vibrio fischeri under transcriptional control of the mutated recN promoter, which is controlled by the bacterial SOS-system (TA 104-recN2-4 strain or Genox strain). After incubation

Last updated on: 26-05-2022 - 10:40

Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal