Dental pulp stem cells (DPSC) are mesenchymal stem cells residing within the inner mucoid core (dental pulp) of teeth, responsible for tissue turnover and regeneration. Since third molars, or wisdom teeth, are frequently extracted for orthodontic reasons, DPSC are a highly accessible stem cell
Last updated on: 23-08-2024 - 11:01
Contact: Nathalie Dirkx
Organisation: University of Hasselt (UHasselt)
Partners: University of Hasselt (UHasselt)
Status: Still in development, History of use, Internally validated, Published in peer reviewed journal
Using an in vitro flow system adhesion assay, immune cell adhesion to (blood-brain barrier) endothelial cells can be quantified under physiological blood flow conditions. Endothelial cells can be treated with inflammatory cytokines or therapeutic antibodies to mimic inflammatory diseases. Immune
Last updated on: 06-06-2023 - 10:35
Contact: Doryssa Hermans
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal
A battery of in vitro genotoxicity assays is currently used to detect agents with DNA damaging and carcinogenic potential. Although the sensitivity of this battery of genotoxicity assays is high, the specificity is low (“false positive hit”), especially with the mammalian cell-based assay, compared
Last updated on: 11-07-2022 - 15:51
Contact: Marc Audebert
Organisation: Institut national de la recherche agronomique (INRAE)
Status: History of use, Internally validated, Published in peer reviewed journal, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
Isolation Femoral and tibial bones are isolated from 10-12 weeks old C57BL/6J mice. After cutting the bone's ends, the bone marrow is flushed out using a 23G syringe with PBS into a 50ml falcon tube. After centrifugation cells are re-suspended in FCS (0.5ml/mouse leg). Differentiation 0.5ml of the
Last updated on: 14-08-2020 - 15:26
Contact: Jerome Hendriks
Organisation: University of Hasselt (UHasselt)
Status: History of use, Internally validated, Published in peer reviewed journal
Characterization of Human Oligodendroglia derived-EV-rich in sHSPs (HOG-EV-sHSPs). Establish HSPB1/B8 stably expressing cell lines for the production of sHSPs rich-EV cell culture conditioned medium. Human Oligodendroglia cell lines (HOG) were stably transduced with pLenti-HSPBs gene constructs
Last updated on: 14-08-2020 - 15:22
Contact: Joy Irobi
Organisation: University of Hasselt (UHasselt)
Status: Still in development, Internally validated, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
Peripheral blood mononuclear cells (PBMC) are isolated from the peripheral blood using Ficoll gradient centrifugation. The PBMC are then stained with fluorescently-labelled monoclonal antibodies directed against cell surface or intracellular proteins that can be used to identify specific immune cell
Last updated on: 03-03-2020 - 11:46
Contact: Cannot be disclosed
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal
Cell line which is commonly used to study biological processes in mouse macrophages. It is a macrophage-like cell line derived from BALB/c mice.
Last updated on: 25-02-2020 - 23:58
TEM, NTA and western blot were used to characterize the purified inflammation‐associated EV subpopulations from TNF-α treated HUVEC based on their sedimentation speeds (10K and110K) and surface markers (CDs and ICAM-1). Protein arrays were used to discover the immunomodulatory content of
Last updated on: 14-11-2019 - 14:02
Contact: Baharak Hosseinkhani
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
This method describes the steps from a living mouse to a single cell solution of primary cortical neurons.
Last updated on: 08-11-2019 - 13:17
Contact: Melissa Schepers
Organisation: University of Hasselt (UHasselt)
Status: Internally validated
This method describes the steps from a living mouse to a single cell solution of primary oligodendrocyte precursor cells.
Last updated on: 08-11-2019 - 13:16
Contact: Melissa Schepers
Organisation: University of Hasselt (UHasselt)
Status: Internally validated